Horse normal serum (2 ml)
Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.
Llama Ig Fraction
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05% (w/v) Sodium Azide> 4.5 mg/ml (E 1% at 280 nm = 13.0)
Rabbit Ig fraction
Rabbit immunoglobulin fraction is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.Rabbit serum can be used
Donkey normal serum (10 ml)
Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot